Development of recombinant proteins

Do you want to develop recombinant proteins? CER Biotech can do this for you.

Obtaining recombinant proteins

CER Biotech provides a large range of molecular biology services to do with recombinant proteins. We could mention cloning and optimisation of expression (constructions, choice of cells, medium, culture conditions), production of bacteria and eukaryotic cells, design of practical tests, to name but a few. Be it transfer of a validated procedure or fine tuning a new method, our experienced team will endeavour to meet your requirements in our ISO 9001:2008 accredited unit.

Constructions

  • Primer design
  • Gene synthesis and construction of the vector
  • Mutagenesis

Cell Banking: Storage and preservation of your clones

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Production in different hosts

  • E. coli, eukaryotic cells
  • Volume of culture available from 1 ml to 7 litres.

Purification

  • Cell lysis
  • Dialysis/ultracentrifugation
  • Chromatography
  • Lyophilisation
  • Ultrafiltration

Quality control

  • Determination of purity (SDS-PAGE, capillary electrophoresis)
  • Immuno-enzymatic tests
  • Functional tests

 

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