Diagnosis of viral diseases

We are most often called on to tackle the following viral diseases:

  • Viral Haemorrhagic Septicaemia or VHS
  • Infectious Haematopoietic Necrosis or IHN
  • Koi Herpes Virus or KHV
  • Infectious Pancreatic Necrosis or IPN
  • Spring Viraemia of Carp or SVC


Using the Elisa antigen and PCR techniques, a rapid diagnosis can be obtained for:

  • VHS
  • IHN
  • SVC
  • KHV
  • IPN
  • Nodavirus or VNN
  • ISA


This is usually done isolating the virus on cell culture. The laboratory has many lines (EPC, RTG2, BF2, BB, FHM, KF, CCB, SSN, EK, WSS) for isolating different viral agents.

         poisson maladiesvirales cellulesepc                                                                                               poisson maladiesvirales epcinfectee                                                          


Culture of EPC cells (epithelioma papulosum cyprini). 

                         

The same EPC cell line infected by the VHS virus isolated from a trout.

This cell line is frequently used for the diagnosis of many viral diseases including Spring Viraemia of the Carp.

The growth of a virus is indicated by the localised destruction (one plaque) of the layer of cells.

It can be identified by an immunoenzymatic reaction that uses specific monoclonal antibodies developed by the Biotechnology Department.

 


The fish pathologies laboratory collaborates with the Department of Immunology of the ULG Faculty of Veterinary Medicine in the KHV programme. See scientific publications.

poisson necrosebranchiale                  

Gill necrosis characteristic of a KHV infection (Koi Herpes Virus).

For more informations , please contact us.

Diagnosis of bacterial diseases

These can be diagnosed simply with a basic stain or the more specific Gram or Ziehl Neelsen stains.

Samples of suspect matter can be inoculated onto general-purpose blood-based culture media or onto the more highly-specific media required to isolate and identify individual species of bacteria (Shieh, SKDM2, etc.).

The Biolog system can be used to assist in identification.

Antibiograms enable us to select the best antibiotic to use.

poisson maladiesbacteriennes nodulestuberculeux              poisson maladiesbacteriennes bacilles       
Tubercle granuloma isolated from the viscera  Acid fast organisms detected (in red) in these
of a zebrafish  nodules after Ziehl-Neelsen stain

 

For more information, please contact us.

Establishment of water quality parameters

The main water quality parameters are systematically determined when consulting.

  • pH
  • Ammoniac
  • Nitrites
  • Oxygen
  • Hardness
  • SBV

If necessary, other parameters can also be determined:

  • Nitrates
  • Phosphates
  • Copper
  • Chlorine
  • Zinc
  • Iron
  • Sulphite
  • Gaz saturation

Some of the parameters need to be tested on the site, others can be tested at the laboratory on the basis of samples provided.

Fore more information, please contact us.

Diagnosis of parasitic and fungicidal diseases

These pathogen agents are identifiable by takings made on fresh smear, on the skin, the gills and the digestive content using an optical microsope.
Some parasitosis can only be determined que post mortem.
The resort to histopathology sometimes allows confirmation of these parasitic infestations.
Technique PCR to diagnose the Aphanomyces astaci, agent responsible for the "plague" in crayfish.

poisson maladiesparasitaires

Spores of Myxosoma cerebralis in the fish bone tissues.
Colouring of Giemsa.

For more informations, please contact us.

Fragmentation of antibodies service

For some therapeutic and imaging applications, fragments of antibodies, particularly scFv, have important advantages compared with conventional monoclonal antibodies from mice:

  • reduction of immunogenicity in humans
  • facilitated penetration of these small molecules into the tissues
  • stable
  • soluble
  • easily produced

The affinity of these fragments with their target is entirely comparable with that of conventional antibodies. Their efficacy has been demonstrated in preclinical models in mice and confirmed in clinical trials in humans.
Whether the hybridoma you desire is already available or has yet to be obtained, we make our expertise available for your project. 

Implementation stages:

Creation of mouse monoclonal antibodies

  • Sequencing of mouse Vkappa and VH chains
  • Synthesis of a gene (using an optimized codon)
  • Cloning in an expression vector
  • Production in E. coli or in eukaryotic cells
  • Quality assessment of the engineered antibody fragments


Contact us