Diagnosis of viral diseases

We are most often called on to tackle the following viral diseases:

  • Viral Haemorrhagic Septicaemia or VHS
  • Infectious Haematopoietic Necrosis or IHN
  • Koi Herpes Virus or KHV
  • Infectious Pancreatic Necrosis or IPN
  • Spring Viraemia of Carp or SVC


Using the Elisa antigen and PCR techniques, a rapid diagnosis can be obtained for:

  • VHS
  • IHN
  • SVC
  • KHV
  • IPN
  • Nodavirus or VNN
  • ISA


This is usually done isolating the virus on cell culture. The laboratory has many lines (EPC, RTG2, BF2, BB, FHM, KF, CCB, SSN, EK, WSS) for isolating different viral agents.

         poisson maladiesvirales cellulesepc                                                                                               poisson maladiesvirales epcinfectee                                                          


Culture of EPC cells (epithelioma papulosum cyprini). 

                         

The same EPC cell line infected by the VHS virus isolated from a trout.

This cell line is frequently used for the diagnosis of many viral diseases including Spring Viraemia of the Carp.

The growth of a virus is indicated by the localised destruction (one plaque) of the layer of cells.

It can be identified by an immunoenzymatic reaction that uses specific monoclonal antibodies developed by the Biotechnology Department.

 


The fish pathologies laboratory collaborates with the Department of Immunology of the ULG Faculty of Veterinary Medicine in the KHV programme. See scientific publications.

poisson necrosebranchiale                  

Gill necrosis characteristic of a KHV infection (Koi Herpes Virus).

For more informations , please contact us.