Purification and coupling of antibodies

Do you want to purify your antibodies? Several methods of purification are available. We will investigate with you which method is best suited to your purity requirements.

Purification service

CER Biotech has the right experience and preparative chromatography equipment to purify monoclonal and polyclonal antibodies and recombinant proteins. Our antibodies are provided dialysed and aliquoted according to your recommendations. A capillary electrophoresis purity check is performed at the end of the process.

machine AKTA

 

Chromatography methods

Depending on the physico-chemical properties of the antibodies, their isotype and their origin (species, matrix, etc.), a variety of purification strategies is applicable:

  • Affinity chromatography: Protein G, Protein A, Nickel sepharose
  • Ion exchange chromatography: anion/cation
  • Hydrophobic interaction chromatography: MEP [Mercapto-Ethyl-Pyridine]
  • Size-exclusion chromatography: gel filtration

Antibody coupling service

Many modifications of antibodies are possible (coupling, fragmentation, etc.) which enables us to obtain effective tools in multiple applications.
We can couple your antibodies to a number of enzymes (HRP, Biotin) and to fluorochromes (FITC)


For more information


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